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Global Research Journal of Microbiology Vol.3(2) pp.18 –23 June 2013.
Available online http://www.globalresearchjournals.org/journal/grjm
Copyright ©2013 Global Research Journals
Full Length Research.
The antagonistic activities of Lactic acid bacteria isolated from Nigerian salad vegetables against methicillin resistant Staphylococcus aureus.
Tajudeen Akanji Bamidelea* ,Bolanle Alake Adeniyib,Funmilola A. Ayenib, Muinah Adenike Foworaa and Stella Ifeanyi Smitha
a Nigerian Institute of Medical Research, Yaba, Lagos State, Nigeria
b Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Oyo State, Nigeria
*Corresponding author at: Department of Molecular Biology & Biotechnology, Nigerian Institute of Medical Research, 6 Edmond Crescent, Yaba, Lagos, Nigeria. Tel. +2348038578093, E mail: deletaju@yahoo.co.uk; ta.bamidele@nimr.gov.ng
| Abstract |
Lactic acid bacteria (LAB) from different niches have been reported to exhibit antimicrobial activities against pathogens including the drug resistant ones. This study investigated the anti- methicillin resistant Staphylococcus aureus (MRSA) activities of LAB isolated from salad vegetables. 0.5 Mc Farland standard (108 CFU/ml) of five confirmed MRSA strains, carrying mecA gene were challenged in the spot streak, agar well diffusion of the cell free supernatant (CFS) and soft agar (0.7%) overlay assays. Thirty- one (31) isolates of LAB from lettuce, cucumber and cabbage procured from eight market locations in Lagos, Nigeria were used. The isolated LAB were as follows; Pediococcus pentosaceus, Weissella confusa (cucumber), Lactobacillus cellobiosus (cabbage), and Lactobacillus plantarum, and Lactobacillus salivarius (lettuce). The degrees of inhibition against MRSA varied in different assays; in the spot streak, P. pentosaceus and L. cellobiosus from Festac and Isolo markets respectively exhibited widest zones of inhibition. In the agar overlay, P. pentosaceus and W. confusa from Mile 12 and Festac markets showed the widest zones of 28 mm and 24 mm respectively while the MRSA exhibited resistance to most of the CFS.
Although the particular inhibitory metabolites have not been elucidated, the LAB from salad vegetables have been found by this study to possess anti- MRSA abilities in- vitro.
Key words: Methicillin resistant Staphylococcus aureus, CFS, LAB, Antagonistic.