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Global Research Journal of Microbiology Vol.1(3) pp.043 – 055 August 2011.
Available online http://www.globalresearchjournals.org/?a=journal&id=grjm
Copyright ©2011 Global Research Journals
Full Lenght Research.
Production of L-glutamic acid from Bacillus isolates cultivated on agro-industrial wastes containing medium.
*Lawal , A.K 1,Oso B.A.2, Sanni, A.I2, Grillo, J.A3 and Elemo.G.N1
*Corresponding Author’s E-mail: Kolakuns@yahoo.com, GSM: 08023164214.
Accepted 14th July 2011
| Abstract |
An investigation was carried out to test the potential of using agro-industrial wastes as fermentation medium for the production of L-glutamic acid.The bacteria strains used for the study included Bacillus subtilis (UG1) from “Ugba”, Bacillus licheniformis (OG1) from “Ogiri”, B.pumilus (DD4) from “Dawadawa” and B.polymyxa (OG7) from “Ugba” and reference strain Corynebacterium glutamicum ATCC 13032.The agro-industrial wastes tested were hydrolysates of corn pomace and cassava peel.The production of L-glutamic acid was done under the single(when pineapple wastes) and double(starch based)stage fermentations.The study showed that pineapple was the best substrate for L-glutamic acid production:i.e the highest production of 6.2mg/ml was for B.licheniformis OG4 while the lowest production of 5.0mg/ml was from B.pumilus DD4.Corn pomace was the least efficient in enhancing L-glutamate production giving 4.9mg/ml from B.licheniformis as the highest and 4.2mg/ml from B.subtilis as the lowest.The rapid consumption of reducing sugar in the pineapple waste medium brought about a drastic fall in the pH to 4.0 at 72h of fermentation and an increase in L-glutamic acid production. Utilization of the agro-industrial wastes for L-glutamic acid will be of immense benefit at preventing the pollutional hazards associated with these wastes.
Keywords: L-glutamic acid, agro-industrial wastes, fermented vegetable proteins, fermentation.