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Global Research Journal of Microbiology Vol.3(1) pp.8 –12 February 2013.
Available online http://www.globalresearchjournals.org/?a=journal&id=grjm
Copyright ©2013 Global Research Journals
Full Length Research.
Aerobic Bacteria Associated With Diabetic Wounds In Patients Attending Clinic In A Rural Community In Nigeria.
Isibor Jonathan Osariemen1, Samuel Sunday Olowu2, Eyaufe Adevbo2, Edomwonyi Edwin Omon3, Odiase Victoria1, Ehiaghe Joy Imuetinyan4, and Eidangbe Adesuwa5.
Departments of ⃰1Microbiology, Faculty of Natural Sciences, 2Medical Microbiology, Faculty of Clinical Sciences, Ambrose Alli University, Ekpoma, Nigeria, 3Orthopaedics and Traumatology, Irrua Specialist Teaching Hospital, Irrua, Nigeria, 4Lahor Public Health and Research Centre, Benin City, Nigeria, and 5Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma.
⃰ Corresponding author’s e-mail: + (234)803 551 5110; joe_isibor@yahoo.com
Abstract
Some studies from within and outside Nigeria have documented the polymicrobial etiology of diabetic wound infections in patients. The need to ascertain the prevalent types of aerobes affecting wounds in diabetic patients in a rural community, informed this investigation. One hundred and fifty (150) wound swabs were collected from diabetic patients attending clinic in Irrua Specialist Teaching Hospital, Irrua. The swabs were inoculated into MacConkey agar, Blood agar and Chocolate agar media. Plates were incubated at 370C for 24 hours and observed for growth. Colonies growing on culture plates were Gram stained and identified using standard bacteriological methods. Antibiotic sensitivity tests were carried out on isolates using the disc diffusion method. The occurrence of bacterial isolates in the wounds investigated was in the following decreasing order of frequency; Staphylococcus aureus (38%), Escherichia coli (24%), Proteus spp (20%), Klebsiella spp (10%) and Pseudomonas aeruginosa (8%). Of the 129 male patients, the percentage isolation rates of pathogens were 18.6, 2.3, 32.6, 30.2, and 16.3, for the respective age ranges of 40-49, 50-59, 60-69, 70-79 and 80-89. The respective rates for the 21 female patients were 0, 4.3, 57.1, 28.6, and 0. All the isolates were resistant to Erythromycin and Cotrimoxazole, while the relative percentage rates of sensitivity of bacterial isolates to antibiotics tested were in the decreasing order of Perfloxacin (13%), Augmentin (10%), Rocephin/Zinnacef (8%), Ciprofloxacin (6%), Gentamicin (3%), Streptomycin (2%) and Amoxicillin (1%). Knowledge of the local isolates from diabetic wounds and their susceptibility pattern would greatly assist in the proper management of patients.
Key words: Aerobes, Diabetic wound, Antibiogram, Rural community, Nigeria.